Fermentation
During
fermentation, lactic acid bacteria provided by the starter culture
preparation utilises sugar within the meat and sugar added to the
batter to produce acid which lowers the pH of the batter and prevents
the growth of pathogenic bacteria. During its growth, the starter
culture also produces flavour compounds, enzymes, and other compounds
which prevent the growth of other bacteria.
The
rate of acid production and pH fall depends on several factors,
including the number and type of lactic-acid bacteria in the starter
culture,
the fermentation temperature and the amount and type of fermentable
sugar. Effective temperature control is critical to the safety of the
final product. The validated fermentation temperature must be
followed, and it must be monitored since deviations can significantly
affect
the lethality of the process and E. colireduction.
It
is important that the product reaches a pH below 5.2 (or other
validated pH) within a specified period to control the growthof
pathogenic microorganisms including Staphylococcus aureus and
pathogenic E. coli. Slow or delayed fermentation may allow the growth
of Staphylococcus aureus, Clostridium botulinumand other bacterial
pathogens. This pH reduction also induces protein coagulation and
reduction in the meat’s water holding capacity which favours water
release and facilitates drying (Toldra, 2006).
In
general, higher fermentation temperatures require shorter
fermentation times, and faster pH falls to prevent pathogen growth.
For example, at the lower temperature range of 21°C to 24°C,
fermentation can take as long as two to three days. At 28°C to 32°C
(range commonly used in NewZealand), 16 to 24 hours of fermentation
is usually required. In the United States, where faster overall
production time are preferred, the fermentation temperature can be as
high as 37°C to 40°C for as little as 12 to 18 hours (Hutkins,
2006).
To
ensure that the pH decreases within the required rate, pH readings
must be taken from each batch during the fermentation period. pH
monitoring is further discussed in section 7.3 of this guide.
Beef
and venison
UCFM
manufacturers who use New Zealand beef or venison can use the
national profiles for E. coli from the National Microbiological
Database (NMD) programme, which is administered by the New Zealand
Food Safety Authority (NZFSA).
Under
the NMD programme, slaughter premises processing cattle, calves,
lamb/sheep, deer, goats, chickens and ostriches, test meat for E.
coli at least weekly, and their data is recorded in a national
database. NZFSA is, therefore, able to provide a national profile
for these meats which is likely to reflect most meat produced in New
Zealand. Manufacturers of UCFM products can simply use the national
profiles and determine the required inactivation of E. coli from
these values.
Table
2 provides E. coli values for beef and venison from the national
profiles as of 30 March 2009. NZFSA recognizes that rare
contamination events can occur that multiply the usual counts by
1000-fold, and therefore the values have been based on the 98th
percentile of national data, not the maximum.
Table
2 also shows the required inactivation based on the 98th percentile.
A value rounded to the nearest whole log count has also been provided
for simplicity. This rounded inactivation number of 2-log includes a
safety margin to try to take account of the measurement of
uncertainty and sampling. Therefore, the validated UCFM process must
be capable of achieving a 2-log reduction in the number of E. coli
when New Zealand beef or venison is used as the raw material.
Table
2: E. coli Count (98th percentile) and Required Inactivation
|
Species |
E.coli (log
cfu/g)
|
Inactivation
number (log)
|
Rounded
inactivation number (log)
|
|
Beef |
1.3 |
1.3 |
2 |
|
Venison |
1.77 |
1.75 |
2 |
Manufacturers
may gain more flexibility for their product or process if they obtain
meat from known suppliers and can use the suppliers’ own NMD data.
For instance, individual suppliers may have an E. coli profile with a
98th percentile less than that of the national profile. Therefore,
the manufacturer may be able to apply a process with a lower
lethality (i.e. less than 2-log reduction).
Manufacturers
who use imported beef will need to obtain E. coli data from their
meat supplier, or do their own tests on their incoming raw materials.
Testing should be based on a sampling plan which will provide
sufficient data over a certain period so that the manufacturer and
regulator can have confidence that the validated process can
consistently reduce E. coli to specified limits. The 98th percentile
of that data should be used to calculate inactivation requirements.
Pork
Pork
has not been previously included in the NMD programme. The
implementation of the NMD for pigs will start in August 2009,
therefore, NMD data is not currently available for domestic pork.
When sufficient data becomes available, manufacturers of UCFM
products can use the national profiles and determine the required
inactivation of E. coli from these values, similar to the approach
discussed for beef and venison.
For
imported pork, and in the absence of NMD data for domestic pork,
manufacturers may use their meat supplier’s own data, when this is
available. The 98th percentile of that data should be used to
calculate inactivation requirements, as discussed in the previous
section. Manufacturers may also do their own tests for their incoming
raw materials. Testing should be based on a sampling plan which will
provide sufficient data over a certain period so that the
manufacturer and regulator can have confidence that the validated
process can consistently reduce E. colito specified limits.
A
recent survey of domestic and imported pork showed that the 98th
percentile of E. colicounts was below 100 cfu/cm2. Data from
Australia and Sweden show similar results. It would therefore be
appropriate for manufacturers who do not have sufficient data of
their own to target a 2-log E. colireduction for the initial
validation of their UCFM process. However, these manufacturers are
expected to do their own tests on their incoming imported pork raw
materials and build up sufficient data, or use the NMD national
profiles for domestic pork when it becomes available. The UCFM
process must be revalidated when results show that the 98th
percentile for E. coli counts is greater than 2-log
Link to Full report
http://www.foodsafety.govt.nz/elibrary/industry/guidelines-production-uncooked-guide/ucfm-guide-july-2009-final.pdf
IMPORTANT
DISCLAIMER
Every
effort has been made to ensure the information in this report is
accurate.
NZFSA
does not accept any responsibility or liability whatsoever for any
error of fact,omission, interpretation or opinion that may be
present, however it may have occurred.
Website
A
copy of this document can be found at:
www.nzfsa.govt.nz/processed-food-retail-sale/standards/oucfm-food/index.htm
Review
of Guideline
This
guideline will be reviewed, as necessary, by the New Zealand Food
Safety Authority. Suggestions for alterations, deletions or additions
to this guideline, should be sent, together with reasons for the
change, any relevant data and contact details of the person making
the suggestion, to:
Assistant
Director (Production and Processing)
New
Zealand Standards Group
New
Zealand Food Safety Authority
PO
Box 2835
Wellington
Phone:
(04) 894 2500
Fax:
(04) 894 2643
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